Chiral Separation of Ferrocene Derivatives by HPLC

β-cyclodextrin bonded silica stationary phase(CDs) were prepared by a facile method via the spacer 3-glycidoxypropyltriethoxysilane(KH-560).The chiral chromatographic properties of CDs were tested.Good resolutions for ferrocene derivatives were obtained with CDs,and their retention mechanism was investigated and discussed.The influence of column temperature for the chiral separation of ferrocene derivatives was also studied on the CDs,and all the tested temperatures were lowered than the isotemperature Tiso.The result showed that there was no evidence that the configuratoin of stationary phase changed,and reservation mechanism and enantiotropy selection action remained unchanged.Δ(ΔH0) and Δ(ΔS0) were both negative which showed that the enantioseparation was under enthalpy control.