Optimized Conditions of 1-Phenylpropanol Separation on the New Chiral Stationary Phase of Peptide Bonding β-Cyclodextrin

A new derivate of β-cyclodextrin with peptides was prepared,where the peptides were obtained from the enzymolysis of bovine serum albumin(BSA) in the presence of trypsin.The derivate was bonded with silica gel to give a novel chiral stationary phase for high performance liquid chromatography(HPLC).The chiral separations of(±)1-phenylpropanol was investigated using an re-phase RP-HPLC system with this column.Several important HPLC conditions were studied including variability of gradient time of pH value of phosphate buffer,ionic intensity,and mobile phase velocity.The chiral recogniztion mechanism and the repetition on chiral separate of 1-phenyl-propanol on the new stationary phase were probed.Experiments shown that the best condition of 1-phenylpropanol on the new chiral stationary phase(CSP) is mobile phase,60 mmol/L phosphate,gradient pH buffer,1 min pH 5.6,10 m in pH 6.4,flow rate,0.5 mL/m in.