Abstract: The expression of Vitreoscilla hemoglobin gene (vgb gene) in Corynebacterium glutamicum ATCC 14067 increased the oxygen and energy supply resulting in improving glutamic acid (Glu) and glutamine (Gln) productivity. Under 5% dissolved oxygen, the cell dry weight (CDW) of the recombinant strain and wild strain were 45.1 g/L and 54.1 g/L respectively, the concentration of Glu were 9.56 g/L and 1.27 g/L, the concentration of Gln were 5.51 g/L and 2.27 g/L. The results indicated that increasing oxygen supply via vgb gene expressing in recombinant C. glutamicum remarkably promote cell growth and production of Glu and Gln. This could be an effective way to reduce the cost of industrial fermentation of Glu and Gln.