红霉素肟主杂质的分离和鉴定

Separation and Identification of the Main Impurity in Erythromycin Oxime

摘要: 深入研究了红霉素的肟化反应. 分离和鉴定了在合成红霉素肟的过程中产生的一种主要杂质, 通过 IR,UV,MS,1 H NMR和 1 3C NMR确证它和红霉素在酸性水解时生成的红霉素 A 8,9-脱水 - 6,9-半缩酮为同一化合物. 这一结果说明, 在酸的存在下, 红霉素的肟化反应总是伴随有它的酸性降解反应. 因此, 在合成红霉素肟时, 严格控制作为催化剂的酸的用量是极为重要的.

Abstract: The oximation of erythromycin is studied. The main impurity involved in the synthesis of erythromycin oxime was separated and purified, which was identified as the very compound formed in the acidic hydrolysis of erythromycin A, i.e erythromycin A 8,9 dehydro 6,9 hemiketal. This result shows that the oximation of erythromycin is always accompanied with its acidic degradation, and therefore that it is important to control the amount of acid used as catalyst.