Abstract: As a key technology for space target detection and localization, microwave measurement technology based on the direct sequence spread spectrum plays a pivotal role in the field of space-based detection. For high-precision measurement requirements in space rendezvous and docking missions, an effective optimization method based on multi-stage filtering was proposed to suppress the measurement fluctuation introduced by the sampling clock jitter in existing active response two-way measurement systems with pseudocode continuous wave transmission. The simulation results show that after multi-stage filtering, the peak of the measurement fluctuation can be reduced by two orders of magnitude and effectively suppressed. The results of measured data collected in a microwave dark room further verify the effectiveness of the optimization method. The implementation of proposed method costs low and can get evident optimization effect, making it suitable for optimizing and upgrading existing active response two-way measurement systems with pseudocode continuous wave transmission.