Abstract: Two-stroke opposed engines are widely used in unmanned aerial vehicles and other fields due to their excellent power-to-weight ratio. The working method of spark ignition makes them more suitable for gasoline as the active medium. Aiming at the technical requirement of using aviation kerosene for equipment development, a test comparison was carried out between different media (kerosene and gasoline) and different injection systems (air-entrained fuel injection and port injection). The results show that when the fuel is the same as gasoline, under other throttle valve opening conditions, the engine power with air-entrainment infusion is better than port injection; only the air-entrainment method can make kerosene realize cold start; In the machine test, under high-speed conditions, the throttle opening is increased from 30% to 80%, and the power and torque are increased by about 10 kW and 65 N·m respectively. The working process is almost the same as a traditional spark ignition engine; Finally, the performance difference between kerosene and gasoline under the air-entrainment mode with the throttle valve controlled at 50% was explored, which showed that the power performance of gasoline fuel was better, and the power and torque were increased by about 5 kW and 35 N·m, respectively.