Abstract: It’s one valuable work to deduce the establishment process of genetic information transmission rule in prokaryote cell from early life origin. According to the physical and chemical condition and evolution existed possibly in early stage after Earth birth, the chemical inevitability of forming small biological molecular and the logical inevitability of forming biomacromolecule and showing clue of life were proposed in this paper. Firstly, based the proposal views of keeping relative balance among those original chemical reactions, the reasons and significance were analyzed for the relative stabilization of biomacromolecule structure, little harm to involved system and its congeries cause of formation. And the forming mechanism of vesicula (structure of cell-like) and its significance were also elucidated. Then, based on template reaction, an inter-chose (Model of Soft-Hard Butt-joint) between the “soft template” (mRNA) and “hard synthesizing structure” (ribosome) was presented in synthesizing protein and the reason was also proposed for many sorts of RNA introduced in protein synthesis. Subsequently a mode of “weak genetic relationship” formed from long time evolution in vesicular was presented. And then, several important scientific topics on the origin of life and evolution were analyzed from the Logic angle, including early or late of DNA and RNA formation, early or late of protein and nucleic acid formation and the relation of Virus evolution and Cell evolution etc. Finally, the logic of life origin and redefinition of life phenomenon etc. were discussed.