Abstract: To further develop lightweight and effective individual stab-resistant equipment, this study drew on the biological armor model of nature to design a bionic stab-resistant substrate with a triangular pyramidal structure. Numerical simulations and experimental testing of a knife impacting a substrate were used to study the impact-resistance behavior and stab-resistance mechanism of a triangular pyramidal stab-resistant substrate, and an optimal structural design was thus obtained. Because the triangular pyramidal structure had a dispersing effect on the knife impact force, the material could avoid the concentrated damage by the impact kinetic energy, giving the triangular pyramidal structure a much higher stab resistance than a comparable flat plate. The optimal angle of the triangular pyramidal stab-resistant substrate produced from a modified titanium alloy (TC611) material with excellent impact toughness was found to be 22.5° and the optimal thickness 1 mm. A full stab-resistant layer designed and manufactured using a new riveting method passed the knife impact test for police stab-resistant clothing in line with the GA 68-2008 standards. This clothing works more effectively against the impact of a knife, being lightweight, and having a shorter manufacturing cycle, and higher wearability.